Fig. 6

MT1-MMP proteolytic activity contributes to invadopodia expansion. a MDA-MB-231 cells expressing Tks5^GFP (green) were plated on top of a thin layer of type I collagen (magenta), treated with the MMP-inhibitor GM6001 and analyzed by live cell imaging as in Fig. 1f (see Supplementary Movie 3). b Color-coded time-projections of five images made at 10-min intervals, corresponding to the boxed region in a. c Elongation rate of invadopodia along collagen fibers in cells treated with GM6001 (GM) compared to mock treatment. Data are presented as the mean from three independent experiments; Mann–Whitney test. n: number of cells; (n): number of invadopodia. d Radial invadopodia expansion rate in cells treated with GM6001 compared to mock treatment. Data are presented as the mean from three independent experiments; Mann–Whitney test. n: cell number; (n): invadopodia number. e Displacement of an invadopodia/collagen-fiber ensemble before and after laser-ablation in mock- (gray) and GM-treated cells (pink) (see Supplementary Movie 4). n: cell number; (n): invadopodia number from three independent experiments. Data were analyzed using two-way ANOVA with Sidak’s multiple comparisons test for each time point. Error bars SEM, **** P < 0.0001, ns not significant