Fig. 2 | Nature Communications

Fig. 2

From: De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns

Fig. 2

Supported membrane assays with de novo synthesized MinD and MinE proteins. a Schematic of the experimental workflow for end-point expression assays. Both minD and minE genes are expressed in a PURE system reaction in the presence of DnaK chaperone mix for 3 h. The solution is then supplemented with 2.5 mM ATP and a trace amount of purified eGFP-MinD (100 nM) before transfer on top of a supported lipid bilayer. b Fluorescence microscopy images of representative types of MinDE dynamic patterns. Videos can be found in Supplementary Movie 1. Scale bars are 20 µm. c Schematic illustration of the in situ MinDE co-expression and self-organization on an SLB. d Several SLB fields of view were imaged at different points during in situ co-expression of MinD and MinE proteins. Corresponding videos are shown in Supplementary Movie 2. Scale bars are 20 µm

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