Fig. 5
Autonomous liposome deformation induced by synthetic Min oscillations. a Time-series images of a liposome that changes morphology in response to Min oscillations. The membrane dye signal is colored in magenta and the eGFP-MinC reporting the waves is in green. The corresponding movie is shown in Supplementary Movie 8. Scale bars are 10 µm. b Fluorescence time traces of the eGFP-MinC intensity localized at the membrane (magenta) and in the lumen (green) of the liposome displayed in a. The time profile of the liposome elongation is overlaid in black and is phase shifted with respect to the amount of eGFP-MinC in the lumen. c Time-lapse fluorescence images of two liposomes exposed to a hypertonic external medium (100 mM sucrose). Liposomes undergo dramatic shape deformation upon recruitment of Min proteins to the inner surface of the lipid bilayer. The force generated by the redistribution of the Min proteins is sufficient to push a membrane septum and remodel adjacent vesicles. The corresponding videos are shown in Supplementary Movie 9. Scale bars are 10 µm
