Fig. 6
Cell-free expressed MinDE(C) regulates FtsZ spatial organization. a Ring-like structures composed of purified FtsZ-Alexa647 and expressed FtsA can assemble on an SLB. The ability of separately expressed MinC and MinD, or co-expressed MinC/D to inhibit formation of FtsA-FtsZ rings was investigated. A representative fluorescence image is shown for each condition. An 8 × 8 fields of view montages are shown in Supplementary Figs. 12–14. Scale bars are 10 µm. b Schematic of the MinCDE and FtsA-FtsZ-coupled SLB assay. c Two time-series images of FtsZ-Alexa647 dynamic patterns. Scale bars are 10 µm. The corresponding videos are shown in Supplementary Movie 10. An 8 × 8 fields of view montage of pattern 1 is shown in Supplementary Fig. 15. d Cytoskeletal pattern of expressed FtsA and purified FtsZ-Alexa647 combined with MinDE. In the absence of MinC, the FtsZ bundle network is maintained but traveling waves of FtsZ-Alexa647 are also visible (see Supplementary Movie 11). Scale bars are 10 µm. e Kymograph of the FtsZ-Alexa647 signal along the magenta line shown in d. The images were preprocessed to filter out small-scale features (Methods)
