Fig. 2
From: Neuronal network dysfunction in a model for Kleefstra syndrome mediated by enhanced NMDAR signaling
Spontaneous electrophysiological activity of control- and KS patient-derived neuronal networks. a Representative image of a control-derived neuronal network on MEAs stained for MAP2 (red) and PAN Axon (green). b Representative raster plot of electrophysiological activity exhibited by control-derived neuronal network at different time points during development. c–f Quantification of network properties as indicated. g, h Raster plot of spontaneous electrophysiological activity exhibited by g control and h KS networks at DIV 28. 6 s of raw data showing a burst recorded from a representative channel are shown for each iPS line. i–l Quantification of network parameters as indicated. m, n Histogram showing the distribution of m the network burst duration (bin size = 100 ms) and n the network inter burst interval (bin size = 1 s). o Quantification of percentage of spike outside network burst and p quantification of coefficient of variability of the inter-burst interval. n = 23 for C1; n = 10 for C2; n = 10 for CMOS; n = 15 for KS1; n = 15 for KS2; n = 12 for KSMOS. Data represent means ± SEM. *P < 0.05, **P < 0.005, ****P < 0.0001, one-way ANOVA test and post hoc Bonferroni correction was performed between controls and KS patient-derived cultures and Mann–Whitney test was performed between two groups. Source data is available as a Source Data file
