Fig. 3
From: Neuronal network dysfunction in a model for Kleefstra syndrome mediated by enhanced NMDAR signaling
Spontaneous electrophysiological activity of neuronal network derived from control- and CRISPR/Cas9-edited iPS cells. a Isogenic line: CCRISPR and KSCRISPR. b Western blot showing the EHMT1 protein levels in iPS cells. c Representative images of iNeurons stained for MAP2 (red) and synapsin 1/2 (green) at DIV 21 (scale bar 5 µm). d Representative raster plots showing spontaneous activity exhibited by CCRISPR and KSCRISPR at DIV 28 on MEAs. Totally, 6 s of raw data showing a burst recorded from a representative channel are shown. e Quantification of relative EHMT1 protein level, n = 5. f Quantification of synapsin puncta in CCRISPR and KSCRISPR iNeurons, n = 9 for CCRISPR; n = 13 for KSCRISPR. g–k Quantification of network parameters as indicated, n = 7 for CCRISPR; n = 12 for CCRISPR. Data represent means ± SEM. *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.0001, one-way ANOVA test and post hoc Bonferroni correction was performed between controls and KS networks and Mann–Whitney test was performed between two groups. Source data is available as a Source Data file
