Fig. 4
From: Neuronal network dysfunction in a model for Kleefstra syndrome mediated by enhanced NMDAR signaling
Effect of AMPA- versus NMDA-receptor blockade on control and KS patient-derived neuronal network activity. a, b Representative raster plots showing 3 min of spontaneous activity from a control (CMOS) and b KS (KSMOS) neuronal networks grown on MEAs at DIV 28. Where indicated, the cells were either non-treated (NT) or treated with 50 µM NBQX to block AMPA receptors or 60 µM D-AP5 to block NMDA receptors. c, d Bar graphs showing the effect of NBQX and D-AP5 on the network burst rate for c control and d KS neuronal networks (CMOS and KSMOS respectively). The values are normalized to the NT data (n = 6 for CMOS and n = 6 for KSMOS in all conditions). e The effect of a 1-hour D-AP5 treatment on CMOS and KSMOS neuronal network activity. After 1 h, the calcium-permeable AMPA receptor blocker Naspm (10 µM) or NBQX were added. Data represent means ± SEM. **P < 0.005, ***P < 0.0005, n = 8 for CMOS and n = 9 for KSMOS, one-way ANOVA test followed by a post hoc Bonferroni correction was performed between conditions. Source data is available as a Source Data file
