Fig. 7
From: Neuronal network dysfunction in a model for Kleefstra syndrome mediated by enhanced NMDAR signaling
NMDAR antagonist MK-801 rescues KS network phenotypes. a MK-801 (1 µM) effect on KSMOS neuronal network activity (DIV 28). After 90 min, Naspm was added. Graph showing the effect of MK-801 (1 µM) treatment on the neuronal network burst frequency for CMOS and KSMOS derived neuronal network 20 min after application. The values are normalized to the nontreated (NT) condition. b Representative raster plot showing the activity exhibited by KS patient-derived neuronal network (KSMOS) grown on MEAs either nontreated or after one week of treatment with MK-801. Six second of raw data showing a burst recorded from a representative channel are shown for the NT and the treated conditions. c–g Quantification of network properties as indicated, n = 8 for CMOS; n = 6 for KSMOS and n = 6 for KSMOS treated with MK-801. h Canonical scores plots based on discriminant analyses for control (i.e., C1, C2, CMOS, and CCRISPR), KSMOS and KSMOS treated with MK-801 (84% correct classification). Discriminant functions are based on using the following network activity parameters: firing rate, network burst rate, network burst duration, percentage of spike outside network burst and coefficient of variability of the inter-burst interval. Group envelopes (ellipses) are centered on the group centroids, n = 50 for controls; n = 6 for KSMOS and KSMOS treated with MK-801. Pie charts visualize accuracy of discriminant analyses functions by showing the relative distribution of lines per a-priory group after reverse testing for group identity. Data represent means ± SEM. *P < 0.05, ***P < 0.0005, ****P < 0.0001, one-way ANOVA test and post hoc Bonferroni correction was performed between conditions. Source data is available as a Source Data file
