Fig. 4: Vesicular trafficking to the endosomal recycling compartment depends on Ift88 function.
From: Glucose homeostasis is regulated by pancreatic β-cell cilia via endosomal EphA-processing
a EphA3 and pEphA3 at the cell surface, mean ± s.d. b Internalized pEphA3, mean ± s.d. c. pEphA3 and EphA3 in the cytoplasm after internalization and before recycling back to the cell surface, mean ± s.d. d EphrinA5 uptake in shIft88 cells. Biotinylated EphrinA5 was visualized with Streptavidin-AlexaFluor 647 (t = 45 min: p = 0.0379; t = 60 min: p = 0.0062; t = 75 min: p = 0.0329 (t test), mean ± s.d.), discontinuous lines show different slopes, on the side dot plots of t = 60 min compared with t = 0 min in both cell lines. e EphrinA5 uptake in Tx- and veh-treated βICKO islets (n = 3, Δ(vehicle): p = 0.0001; Δ(treated): p = 0.0132; vehicle t = 60 min vs treated t = 60 min: p = 0.0001 (t test), mean ± s.d.). f c-Myc-EphA3 (red) localization in shIft88 expressing MIN6m9 cells with or without Ephrin A5-FC (1 h prior experiment). Scale bar 5 µm.
