Fig. 7: Tiam1/Rac1 misregulation inhibits insulin.
From: Glucose homeostasis is regulated by pancreatic β-cell cilia via endosomal EphA-processing
a Protein and mRNA levels of Tiam1 in isolated βICKO islets treated with tamoxifen and vehicle and MIN6 cell lines, mean ± s.d. b Tiam1 (green) and GM130 (Cyan) localization in shIft88 expressing MIN6m9 cells with Pearson coefficient (p < 0.0001, t test, mean ± s.d.). Scale bar 20 µm. c Dynamic insulin secretion of isolated βICKO islets treated with tamoxifen (blue) or vehicle (ethanol; red) treated with 20 μM NSC23766. On the right, quantification of first phase (AUC, time 18:00 to 30:00 min) and total secretion (AUC, from 18:00 to 48:00 min) (islets pooled from n = 10 animals, experiment repeated n = 4, and insulin measured n = 2 per experiment), in gray Tx-treated βICKO islets and in black vehicle-treated βICKO islets from Fig. 1f for comparison, mean ± s.d. d Biotinylated EphrinA5-Fc uptake in derivative MIN6m9 cells treated with NSC23766 and vehicle. (n = 4, t = 30 min: Control vs shIft88 p = 0.0044, Control vs shIft88 + NSC23766: p = 0.0289; t = 60 min: Control vs shIft88 p = 0.0006, Control vs shIft88 + NSC23766 p = 0.1466 (ANOVA), mean ± s.d.
