Fig. 3

SST-Cre-lineage CINs adopt fast-spiking (PV-like) properties after Tsc1 deletion. a Fluorescence (top) and DIC (bottom) images illustrating that recordings were obtained from tdTomato+/SST+ CINs. Scale bar is 20 µm. b Example voltage traces in response to subthreshold current injections in WTs (gray), Tsc1 cHets (blue) and Tsc1 cKOs (red). c Comparison of resting membrane potential (RMP), (F_{2, 67} = 0.41, P = 0.66) and input resistance (R_in), (F_{2, 67} = 6.49, P = 0.002; WT vs. cKO p = 0.001), One-way ANOVA with Tukey’s test. d Example traces in response to suprathreshold current injections. Traces from two different cells are shown. e Example traces showing action potentials (APs). f Rheobase of SST+ CINs, One-way ANOVA with Tukey’s test (F_{2, 67} = 5.06, P = 0.009; WT vs. cKO, p = 0.006). g Number of APs fired. Two-way ANOVA with Tukey’s test, Genotype: (F_{2, 1320} = 26.8, P < 0.0001); WT vs. cHet, and WT vs. cKO, p < 0.0001; cHet vs. cKO, p = 0.008). h Maximum firing frequency of SST+ CINs from WT, cHet and cKO mice, and PV+ CINs from WT mice (gray triangles) is plotted against the coefficient of variance (CV) of inter-spike interval. Inset, proportion of SST+ CINs with regular-spiking (RS) and fast-spiking (FS) properties. i–m Comparison of AP amplitude (i): (F_{2, 67} = 0.67, P = 0.51), AP threshold (j): (F_{2, 67} = 3.48, P = 0.03; WT vs. cHet p = 0.03), AP max dV/dt (k): (F_{2, 67} = 6.39, P  = 0.002; WT vs. cHet p = 0.004, WT vs. cKO p = 0.009), AP half-width (l) (F_{2, 67} = 11.04, P  < 0.0001; WT vs. cHet p = 0.0001, WT vs. cKO p = 0.001); and fAHP amplitude (m): (F_{2, 67} = 5.27, P = 0.007; WT vs. cKO p = 0.005); One-way ANOVA with Tukey’s test. WT, n = 22 cells, 4 mice; cHet, n = 21 cells, 3 mice; cKO, n = 27 cells, 4 mice; data are presented as mean ± S.E.M. *p < 0.05; **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file