Fig. 3
From: An MPER antibody neutralizes HIV-1 using germline features shared among donors
Dominant role of CDRH3 in PGZL1 HIV-1 neutralization by D-gene-encoded residues. a Fold decrease in neutralization (IC50) of isolate 92TH021 relative to wild-type PGZL1 by CDR grafts and LC substitution from 4E10 (left), and by 4E10 substitutions L100cF and PGZL1 LC (right). b Neutralization (log IC50) of a six-virus panel by PGZL1 and 4E10 variant antibodies. c Effect of Ala substitutions in DH-encoded residues W99 and F100 on the ability of PGZL1 mature (top panels) and inferred germline antibodies (bottom panels) to neutralize Du156.12 and HxB2 (left panels), as well as to bind MPER peptide in an ELISA (right panels). d Antibody polyreactivity in an ELISA as a function of area under the curve (AUC) of PGZL1 and 4E10 variant antibodies against nonspecific antigens. VRC01 is a negative control. Two-way ANOVA multiple comparisons was used to compare the difference between groups (n = 7, **p = 0.0062, ***p = 0.0001, ****p < 0.0001). e Immunofluorescence staining of HEp-2 cells. Antibodies were tested at 50 μg/ml using 4E10 and VRC01 as positive and negative controls, respectively; images are at ×200 magnification and the scale bar is 400 µm. Source data for b–d are provided as a Source Data file.
