Fig. 2 | Nature Communications

Fig. 2

From: Conditional quorum-sensing induction of a cyanide-insensitive terminal oxidase stabilizes cooperating populations of Pseudomonas aeruginosa

Fig. 2

Regulation of cioA (PA3930) by QS is conditional on the presence of cyanide. a Expression of cioA (PA3930) and PA4133 in the WT PAO1 (black, “WT”) and LasR-RhlR-double mutant (white, “QS-”). Solid, in the presence of 150 μM KCN; grid, in the absence of 150 μM KCN. b cioA, PA4129, PA4131, PA4133, and rhdA expression from either WT PAO1 (“policing”, black) or RhlR-null cooperators (“non-policing”, white) when grown in co-culture with cheaters, with the populations separated by dialysis membranes. Expression was measured by RT-PCR with proC used as reference gene. Means, s.e.m., and individual biological replicates are shown (n = 3). Statistical significance determined by unpaired Student’s t-test; ***p < 0.001. UD, undetected

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