Fig. 5

IL-27R deficient HSCs fail to induce AAA. a Scheme of experiment. Apoe^−/− CD45.1-recipient mice were lethally irradiated and reconstituted with donor mixes of Apoe^−/− CD45.1 and Apoe^−/−Il27ra^−/− CD45.2 total BM cells in a 90%:10%, 50%:50%, and 10%:90% ratio, respectively. Four weeks after reconstitution mice were placed on WD for 12 weeks and infused with Ang II for last 4 weeks of feeding. b Percentage of AAA incidence among Apoe^−/− CD45.1-recipient mice receiving BM mixture in a ratio: 90%Apoe^−/−:10%Apoe^−/−Il27ra^−/− (n = 7), 50%Apoe^−/−:50%Apoe^−/−Il27ra^−/− (n = 13), or 10%Apoe^−/−:90%Apoe^−/−Il27ra^−/− (n = 6). *p < 0.05, Fisher’s exact test (two-sided). c Survival curves for Apoe^−/− CD45.1-recipient mice receiving BM mixture in a ratio: 90%Apoe^−/−:10%Apoe^−/−Il27ra^−/− (5/7 died), 50%Apoe^−/−:50%Apoe^−/−Il27ra^−/− (2/13 died) or 10%Apoe^−/−:90%Apoe^−/−Il27ra^−/− (0/6 died) during 28 days of Ang II infusion. *p < 0.05, long-rank test. Proportion of live donor-specific cells in Apoe^−/− CD45.1-recipient mice (n = 5) receiving BM mix in 50%Apoe^−/−:50%Apoe^−/−Il27ra^−/− ratio. d LSK cells, including LT-HSC and HPC-1; e myeloid progenitors, including CMP and GMP (Lin⁻Sca-1⁻c-kit⁺CD16/CD32⁺CD34⁺⁾ in the BM. Proportion of donor-specific mature Ly6C^hi, Ly6C^low-monocytes, and Ly6G⁺ (neutrophils) in the spleen (f), blood (g), and suprarenal aorta (h). Data are mean ± SEM from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.005, unpaired Student’s t-test (two-tailed)