Fig. 6

IL-27R regulates the quiescence of HSCs in Ang II-induced myelopoiesis. LT-HSCs were FACS-sorted from the BM of Apoe^−/−(n = 2), Apoe^−/−Il27ra^+/− (n = 4), or Apoe^−/−Il27ra^−/− (n = 3) mice fed with WD for 12 weeks and infused with Ang II or PBS for last 2 weeks of feeding, and subjected to whole transcriptome analysis (RNA-seq). a Overall number of genes whose expression is changed between Apoe^−/− and Apoe^−/−Il27ra^−/− mice either treated with Ang II (587 genes) or with PBS (control, 16 genes). b Deregulated upstream regulators whose targets were significantly overrepresented (p < 0.05, Fisher’s exact test) among genes uniquely affected by genetic IL-27R deficiency. c Various pathways significantly deregulated (p < 0.05, GSEA) by IL-27R deficiency, as determined by Reactome pathway enrichment analysis. Changed pathways include cell cycle, proliferation, and division in LT-HCS after Ang II infusion (^#p = 0.056, GSEA)