Fig. 3
From: Quorum sensing modulates the formation of virulent Legionella persisters within infected cells
Intracellular L. pneumophila subpopulations produce distinct proteomes. a A. castellanii amoebae were infected (MOI 1, 24 h) with L. pneumophila/Timer, lysed and the intracellular bacterial subpopulations FACS-sorted according to the Timer green/red color ratio. Comparative proteomics of sorted growers (G) and nongrowers (NG) revealed differentially produced proteins. Protein abundance in each subpopulation is depicted as volcano plot (see Supplementary Data 1 for the full dataset). b Functional classification of proteins identified in intracellular growing and nongrowing L. pneumophila subpopulations. The abundance of c Icm/Dot T4SS subunits and d Icm/Dot-translocated effector proteins are depicted. NG nongrowers, G growers. Data represent the mean ± SEM of four biological replicates (n = 4) pooled into two to increase the number of peptides detected
