Fig. 6
From: Quorum sensing modulates the formation of virulent Legionella persisters within infected cells
L. pneumophila produces intracellular nongrowers in macrophages. Naive and IFN-γ treated murine macrophages were infected (MOI 1, 24 h) with a, b L. pneumophila/Timer c, d L. pneumophila/PflaA-gfp or e L. pneumophila/PsidC-gfp, and a, c analyzed by confocal microscopy or b, d, e lysed and analyzed by flow cytometry. Micrographs show the overlay of Timer fluorescence (500 and 600 nm) and DAPI staining (nuclei). White arrows indicate intracellular nongrowers with low green/red Timer color ratio or producing GFP. Scale bars 10 μm. The histograms show the proportion of b intracellular growers (G) and nongrowers (NG) or d, e intracellular GFP producers (GFP+) and nonproducers (GFP-). Data represent the mean ± SEM of three biological replicates (n = 3; light gray filled circles). Student’s t test two-tailed. ***P < 0.001. Source data are provided as a Source Data file
