Fig. 1
The hybrid sequencing workflow for FLT annotation. a The flow chart of the hybrid sequencing workflow, including the experimental procedures (blue rectangles) comprised of PacBio sequencing and Illumina sequencing, and the computational components (pink rectangles) for Illumina-based PacBio error correction, followed by full-length transcriptome reconstruction, transcript end validation, and isoform diversity characterization. b The ratio of PacBio read count to gene expression level (estimated based on Illumina sequencing data) was plotted against the gene expression level. Highly expressed transcripts were largely depleted in PacBio sequencing after the cDNA normalization. c Histograms compare PacBio read accuracy before and after the error correction. After the correction, the final sequence accuracy was increased from 88.55% to 99.76%. The colors filled in the histograms are half-transparent, so that dark red in the histograms indicates the overlay. d Bar plots showing the percentage of alignable reads before and after error correction (72.8% vs. 98.2%). e The increased sequence accuracy after error correction was plotted against gene expression levels. Box edges represent quartiles, whiskers represent extreme data points no more than 1.5 times the interquartile range. Source data for panel b are provided in a Source Data file
