Fig. 3

Integrative analysis of oxidative stress-regulated phospho-signalling networks. a A network diagram visualising the crosstalks of redox- and phospho-signalling. Oxidized cysteine sites (green squares) and phosphosites regulated by oxidation (red circles), insulin (grey circles) or both (purple circles) on key kinases and substrates of Akt, mTOR and AMPK signalling pathways were highlighted. b Heatmaps of standardised phosphorylation levels of Akt, mTOR, and AMPK substrates. Those that were significantly altered by oxidation and/or insulin were highlighted. c Boxplots for phosphorylation changes of Akt, mTOR and AMPK substrates with either 1 or 2 h BCNU/AF treatments vs controls in the presence of insulin. Boxes capture lower quartile and upper quartile with median displayed as a horizontal line in the middle; whiskers are min and max. A one-sided Bartlett Test was used to compare if the variance in (2 h BCNU/AF) was greater than (1 h BCNU/AF) treatment, following insulin stimulation. A one-sided t-test was used to compare if the mean was less than 0 (p < 0.05).