Fig. 6

Reversible in vivo condensation of Aip5 under stress condition. a Representative maximum Z-projection images of in vivo formed Aip5-GFP condensates under 1 h of energy-depletion treatment and followed by replenishment of 2% glucose. The scale bar represents 5 µm. b Representative maximum Z-projection fluorescence images of Abp140-3GFP revealed actin cable pattern in WT and spa2Δ, with or without 1 h of energy depletion. Scale bar represents 3 µm. c–e Quantification of the Abp140-3GFP marked actin cables and actin patches that were imaged in b under energy-depletion treatment for 1 h (cells for analyzing actin cable number: n = 59 for WT and spa2Δ; Actin cable length: n = 99 for WT and n = 45 for spa2Δ; cells for analyzing actin patch number: n = 87 for WT and n = 70 for spa2Δ). f Yeast growth assay on YPD plate with 3.1 mM 2-deoxy-d-glucose and 0.31 µM antimycin A for 72 h at 30 °C. g Representative maximum Z-projection images of Abp140-3xGFP-labeled actin cables from the indicated strains after 1 h of energy depletion and recovery with replenishment of 2% glucose. Scale bar represents 5 µm. WT wild type. p Values were determined by two-tailed Student’s t test assuming equal variances, ****p < 0.0001. All the bar graphs represent mean with an error bar, S.D. Source data are provided as a Source Data file