Fig. 2
ADSL plays an essential role in TNBC. a ADSL mRNA expression across different subtypes of breast cancer in three different datasets (TCGA stands for The Cancer Genome Atlas). The center line of the dox plots represents the median, the bounds the upper and lower quartiles, and the whiskers the 1.5× interquartile range. b ADSL mRNA expression in TNBC and normal breast tissue in TCGA, and IB of lysates from paired TNBC patient-derived non-tumor (n) and tumor (t) breast tissues. c Representative 2-D proliferation of indicated cells upon ADSL depletion shown by IB. d, e Representative images of (d) anchorage-independent growth and (e) invasion of MDA-MB-231 cells infected with either ADSL sgRNAs #5 and #6 (sg5 and sg6) or sgRNA control (Ctrl). Graphs represent the mean ± SEM from two independent experiments, each performed in duplicate (d), and from three independent experiments (e). **P < 0.01 were calculated using one-way ANOVA followed by Dunnett’s multiple comparison test. f IB of lysates from MDA-MB-231 cells overexpressing doxycycline (dox)-inducible ADSL, infected with indicated virus and treated as indicated. g, h Representative images of (g) anchorage-independent growth and (h) invasion of MDA-MB-231 cells overexpressing dox-inducible ADSL, infected and treated as indicated. Graphs represent the mean ± SEM from four independent samples. *P < 0.05, **P < 0.01, ***P < 0.001 were calculated using one-way ANOVA followed by Tukey’s multiple comparison test. Source data are provided as a Source Data file
