Fig. 3
ADSL is required for TNBC tumorigenesis and lung colonization. a Representative bioluminescence images from the indicated weeks after the orthotopical injection of MDA-MB-231 luciferase-expressing cells infected with either ADSL sgRNA #6 (sg6) or control sgRNA (Ctrl) into the mammary fat pads of NOD SCID Gamma (NSG) mice. b Quantification of the bioluminescence imaging. c Representative images of tumors after dissection and quantification of tumor weight. d Representative bioluminescence images from the indicated weeks after the injection of MDA-MB-231 luciferase-expressing cells infected with either ADSL sgRNA #6 (sg6) or control sgRNA (Ctrl) into the tail vein of NSG mice. e Dot blot representation of the raw luminescence radiance in the indicated weeks after injection. f Quantification of the bioluminescence imaging. For (b), (e), and (f), the Mann-Whitney test was used to calculate the P values. For (c), two-tailed Student’s t-test was used. Error bars represent SEM, *P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file
