Fig. 4

JIL-1 and not H4K16ac is responsible for the enrichment of JASPer at the male X chromosome. a Genome browser profile showing mean (n = 3, for MSL3 n = 2) spike-in ChIP-seq normalized coverage in control male S2 cells and after jil-1 RNAi treatment from top to bottom for JASPer, MSL3, H4K16ac and H3K9me2 along representative 200 kb windows on chromosome 2R and X. HAS are marked by red bars above the gene models in gray. b Genome browser profile as in a showing mean (n = 3, for MSL3 n = 2) spike-in ChIP-seq normalized coverage in control male S2 cells and after jil-1 RNAi treatment, from top to bottom for JASPer, MSL3, H4K16ac, and H3K9me2 along a representative 200 kb window on chromosome X. Signal overlay is marked in grey. c Density plot showing mean (n = 3, for MSL3 n = 2) spike-in ChIP-seq normalized coverage in control male S2 cells and after jil-1 RNAi treatment at active (tpm > 1) genes for JASPer (top left), MSL3 (top right), H4K16ac (bottom left), and H3K9me2 (bottom right). X chromosomal genes (n = 1214) are represented by a solid line and autosomal genes (chromosomes 2L, 2R, 3L, and 3R, n = 5785) by a dashed line.