Fig. 5
From: AMPA receptors in the synapse turnover by monomer diffusion
TARP2 dynamically forms metastable complexes with GluA1. a The distributions of homodimer durations of ACP-GluA1 in the HEK293-PM with (magenta) and without (gray, see Fig. 3d) TARP2-mGFP overexpression (expression levels 47 (±30) times higher than those of ACP-GluA1; n = 13 cells). b Typical single fluorescent-molecule image sequences, showing that a TARP2-mGFP monomer (green arrowheads) formed a transient heteromer with an ACP-GluA1 monomer (magenta arrowheads) in the HEK293-PM (a typical result from 358 independent movies). Cyan arrowheads indicate the heteromer. c The duration distributions of the heteromers of TARP2-mGFP with ACP-GluA1 monomers (left) and dimers (right) in the HEK293-PM. The histograms could be fitted well with single exponential decay functions, providing the heteromer lifetimes, which are shown in parentheses (after correction for the probe photobleaching lifetimes). Related ACP-GluA2 results are shown in Supplementary Fig. 10. For statistical parameters, see Supplementary Table 7
