Fig. 4

Public features of clonotypes with more than one isolate from our dataset. a A heat map illustrating 9 groups (I–IX) of cells from multiple subjects sharing identical paired TRBJ/TRAJ genes. Number of isolates of the same clonotype is proportional to the color intensity. Tetramer specificity is differentiated by colors. Tetramer categories without any hit on the heat map are not shown. b Frequencies of V or J genes used in 57 TCRα/β clonotypes with multiple isolates compared with “All Wells” that return sequences (see Supplementary Data 2) or “All Cells” with productive α/β CDR3 sequences (see Supplementary Data 3). Arrows indicate genes used in TRBV29–1_TRBJ2-5/TRAV6_TRAJ24-expressing cells, with fold increase compared to “All Cells”