Fig. 2
From: In-cell identification and measurement of RNA-protein interactions
incPRINT measures cellular RNA–protein interactions. a Interaction intensities detected between Xist(A)-MS2 and the indicated factors, with and without RNase treatment. Data from two biological replicates are presented as mean ± s.d.; RLU are relative light units. b Interaction intensities between Xist(A)-MS2 and the indicated factors. In-cell interaction values were measured in a standard incPRINT experiment. In vitro interaction values resulted from separate transfections of the tagged proteins and Xist(A)-MS2 RNA, which were combined for interaction analyses after the cells were lysed. Data from two biological replicates for each experiment are presented as mean ± s.d.; RLU are relative light units. c Scatter plot showing the correlation of the Xist(A)-MS2 interaction intensities determined by NanoLuc luciferase luminescence from two biological replicates. Shown are median-normalized values. Squared Pearson correlation coefficient is indicated. d Scatter plot showing the correlation of FLAG-tagged protein expression levels determined by anti-FLAG ELISA from two biological replicates. Shown are median-normalized values. Squared Pearson correlation coefficient is indicated. e Scatter plot showing RNA–protein interaction intensities and protein expression levels. RLU are relative light units.
