Fig. 4
From: In-cell identification and measurement of RNA-protein interactions
Proteins identified to interact with distinct regions of the lncRNA Xist. a Schematic representation of the mouse Xist transcript and its A- to F-conserved repeat regions. Exons are indicated as boxes, introns as lines. A zoom-in image of the 5′ region of Xist shows the positions of Xist fragments along the Xist transcript. The 0.9-kb, 2-kb, and 1.7-kb fragments for Xist(A), Xist(F), and Xist(C), respectively, used in the incPRINT experiments are delimited by horizontal colored bars. b Normalized Xist(A)-protein interaction intensities averaged from two biological replicates, sorted in increasing order. The horizontal dotted line represents the interaction intensity cutoff used for the classification of Xist(A) interactors. Red dots are Xist(A)-interacting proteins; gray dots are proteins that do not bind to Xist(A). Selected proteins known to bind to Xist are indicated. See the ‘Methods’ section for data normalization. RLU are relative light units. c As in (b), for Xist(F). d As in (b), for Xist(C). e Heatmap showing interaction intensities between Xist(A), Xist(F), and Xist(C). Previously detected Xist-interacting proteins are indicated on the right. RLU are relative light units. f As in Fig. 3b, for Xist(A), Xist(F), and Xist(C) interaction partners. SPOC refers to Pfam domain PF07744.
