Fig. 4

Controlled fabrication of fluorescent anisotropic nanoparticles by MORPH. a Non-fluorescent seed worms with A:T molar ratio 0.33 were converted into green and then yellow fluorescent nanoparticles by adding BODIPY-FL (PA^G) and -TR (PA^R) tagged polymers sequentially. b–d Confocal fluorescence images (overlay of green and red channels, scale bars = 10 μm) with photographs of the nanoparticle solutions under UV light as insets. e–g Stain-free TEM images confirming the controlled morphological transformation (scale bars = 200 nm).