Fig. 3
Synergistic depolymerization across species and effect of chemical environment. a Rates (±sd) of depolymerization in the presence of 5 μM human ADF or Cofilin-1 and/or 0.83 μM N-CAP1. Statistical comparison: ns no evidence for significance at p = 0.05 by two-sample t test against Srv2 + Cof1 condition. Right, magnified view. *statistical comparison by two-sample t test against Control (p < 0.05). Number of filament ends analyzed for each condition (left to right): 144, 103, 37, 130, 128, 24 and 103. b Rates (±sd) of pointed-end depolymerization by 1 μM Cof1 and 0.5 μM N-Srv2 in absence and presence of 20 mM Pi and 20 mM K2SO4. Statistical comparison: ns no evidence for significance at p = 0.05 between the indicated conditions by two-sample t tests. Number of filament ends analyzed for each condition (left to right): 73, 42 and 27. Source data are provided as a Source Data file. All experiments were performed at least three independent times, and yielded similar results. Data shown are from one experiment.
