Fig. 5
From: Glycerol-3-phosphate mediates rhizobia-induced systemic signaling in soybean
Glycerol-3-phosphate accumulates in the vascular exudates of soybean plants in response to incompatible rhizobia. a G3P levels in petiole (Pex) or root (Rex) exudate of Rj2 rfg1 plants 24 h post inoculation with buffer (MgCl2), compatible (U257), incompatible (U122) rhizobia, or Psg avrB (avrB). Asterisks denote significant differences from MgCl2 of corresponding genotype, Student’s t-test, P < 0.0001 (n = 4). b, c Psg Vir counts presented as LOG10 values of colony forming units (CFU) ^ cm2-1 leaf area from infected leaves at 0 and 4 days post inoculation (dpi). Error bars indicate standard deviation (n = 4). Asterisks denote data significantly different from corresponding MgCl2, Student’s t-test, P < 0.001. b Pex or Rex from buffer (Pex/RexMgCl2), U122 (RexU122), or Psg avrB (PexavrB) inoculated Rj2 rfg1 plants was leaf-infiltrated in a fresh set of Rj2 rfg1 plants followed by Psg Vir inoculation on systemic leaves 48 h later. c Untreated (control), boiled, or proteinase K-treated (Prot K) Rex MgCl2/U122 was leaf-infiltrated in A. thaliana plants (Col-0) followed by Pst Vir inoculation on systemic leaves. d G3P levels in Pex or Rex of V and SilG3Pdh Rj2 rfg1 plants 24 h post inoculation with MgCl2, avrB, or U122. Letters denote significant differences based on two-way ANOVA in SAS (P < 0.05 after Tukey correction). e Psg Vir counts presented as LOG10 values of (CFU) ^ cm2-1 leaf area from infected leaves at 0 and 4 dpi. Error bars indicate standard deviation (n = 4). Asterisks denote data significantly different from corresponding mock, Student’s t-test, P < 0.001. RexMgCl2/U122 from V (plants infected with control VIGS vector) or SilG3Pdh (GmG3Pdh knockdown) Rj2 rfg1 plants was leaf-infiltrated in wild-type Rj2 rfg1 or rj2 rfg1 plants followed by Psg Vir inoculation on systemic leaves. Results are representative of two-four independent experiments.
