Fig. 2
ZFP161 is enriched at replication forks. a Cells were depleted of RPA32 using shRNA. The co-localization of phospho-RPA32 (S4/S8) and ZFP161 were determined by immunofluorescence. b RPA32 deficient HCT116 cells were incubated with EdU and HU. Replication fork proteins were isolated by iPOND and immunoblotted with indicated antibodies. c Cells were depleted of ZFP161 using sgRNA. The co-localization of RPA32 and γH2AX were determined by immunofluorescence. d ZFP161 deficient HCT116 cells were incubated with EdU and HU. Replication fork proteins were isolated by iPOND and immunoblotted with indicated antibodies. e Purified GST-ZFP161 protein and RPA complex were incubated with biotin labeled single fork DNA. The proteins retained on DNA were determined by immunoblotting. Scale bars, 10 µm. Source data are provided as a Source Data file.
