Fig. 6
From: A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion
Microenvironmental oncogenesis attenuates crypt fixation. a Vibratome cross-sections of MCATVilCre and ROBOVilCre small intestines were confocal imaged and tiled for each lineage reporter (PND17). b Higher magnification of areas indicated in “a”. c Whole-mount confocal imaging of small intestines for each fluorescent lineage reporter in ROBOVilCre mice. d Whole-mount confocal imaging of MCATVilCre small intestines. e Indicated callouts (1–8) from “c” at higher magnification. f The percentage of each crypts that are monoclonal (crypt fixation), biclonal, or triclonal was calculated for NCATVilCre mice (N = 3 and 938 crypts), MCATVilCre mice (N = 9, and 6006 crypts analyzed at PND17) and ROBOVilCre mice (N = 6 and 3557 crypts analyzed). (SEM included for each graph). g Wholemount confocal imaging of ROBOVilCre colon at PND18. h Region of interest in “g” at higher magnification. Arrows show examples of crypts where fixation has not occurred. Scale bars = 1 mm in a, 100 µm in b/d, g, h, 200 µm in c, and 40 µm in e. Source data are provided as a “Source Data file”.
