Table 1 Overview of cancer rainbow (Crainbow) mouse lines.

NCAT

N-terminal truncated ßcatenin

Cre Off: Mars1 FAP

Cre On:

1. ΔNßcat

2. ΔNßcat

3. ΔNßcat

The positional recombination efficiency of Crainbow is biased 2.3-fold at position 1, 0.36-fold at position 2, and 0.32-fold at position 3.

Supplementary Fig. 4, Supplementary Data 1

MCAT

Multiple isoforms of ßcatenin

Cre Off: Mars1 FAP

Cre On:

1. ΔNßcat

2. Ccat/Lef1

3. ΔNßcatΔC

Clone expansion occurs rapidly during perinatal development but is constrained by intestinal epithelial homeostasis in adult.

Supplementary Fig. 5, Supplementary Data 2

ROBO

RSPO3 Crainbow

Cre Off: Mars1 FAP

Cre On:

1. RSPO3

2. PTPRK^e1:RSPO3^e2–5

3. PTPRK^e1–7:RSPO3^e2–5

RSPO3 oncogenes expand the crypt microenvironment to induce crypt fission and promote rapid oncogenic clone spreading in the adult epithelium.

Supplementary Fig. 7, Supplementary Data 3