Fig. 2
Endothelial cell migration assay and crystal structure of MYDGF-Fab8 complex. a Migration assay quantifying HCAEC monolayer recovery 16 h after scratch injury in the absence (control) or presence of vascular endothelial growth factor A (VEGFA, 50 ng/ml) or wild-type MYDGF (100 ng/ml) in the absence or presence of control IgG (10 µg/ml), or Fab8 (at indicated concentrations). Fab8 shows neutralizing effects. **P < 0.01 vs. control; #P < 0.05 vs. MYDGF + IgG; n = 3; one-way ANOVA + Dunnett's; SEM error bars. Supplementary Fig. 11 shows the endothelial cell migration assay using Ab8. b Cartoon and surface representation of MYDGF (gray) and neutralizing Fab8 variable light (blue) and variable heavy chain (turquois). c Asymmetrical binding of Fab8 to MYDGF sterically blocks a large area on MYDGF top and front face for binding of the unknown receptor (same color coding as above). d The MYDGF-Fab8 epitope is colored in dark red. All residues, which are possibly shielded by the Fab8 and therefore not accessible for receptor recognition, are colored in salmon.
