Fig. 6

MSL associated CLAMP near BEAF+ and CP/CH+ boundaries. a Considering domain boundaries near active genes, 4C-seq metaprofiles were created for BEAF + and CP/CH + boundaries for each probe obtained from Ramirez et al.⁸. The mean 4C-seq enrichment around domain boundaries (±7 kb) is reported (y axis) for both S2 and Kc cells (***Wilcoxon p-value < 0.0001). b For each BEAF + and CP/CH + domain boundary near active genes, the nearest (max distance ≤ 10 kb) MSL associated CLAMP binding site was considered and its normalized maximum enrichment peak reported in the boxplot for S2 and Kc cells. The CLAMP binding strength in S2 is significantly higher (*** Wilcoxon p-value ≤ 0.001). For each box in panels (a) and (b) the median is marked as horizontal line, the boxes mark the interquartile range (IQR), the whiskers extend up to 1.5 IQR, all individual data points are shown. c Considering BEAF+ and CP/CH+ boundaries near active genes, average pairwise interaction profiles were created in 3.5 kb binned male and female Hi-C maps using a window of 70 kb (20 bins in 3.5 kb Hi-C maps) around boundaries (left). The male chrX average pairwise interaction profile showed noticeably higher signal. The average pairwise interaction profiles were recomputed using a window of 35 kb (10 bins in 3.5 kb Hi-C maps) and the difference (male–female) between the two average interaction profiles is shown (right). The male interaction profile shows higher interaction near the centre. d Using a ±2 bins window around the centre (similar to panel (c)), the average differences in the pairwise interactions between domain boudaries are shown for each autosome arm and chrX. Error bars are mean±standard error of the mean (SEM). For each chromosome, n is as follows: 2L = 28458; 2R = 41080; 3L = 30904; 3R = 68970; X = 46112. e Considering BEAF+ and CP/CH+ boundaries near active genes, the log₁₀ insulation scores for each boundary is shown for chrX (bottom) and chr3R (top) for both male (x-axis) and female (y-axis) embryos. The observed linear fit (red) and expected (y = x, black dashed line) relationship between male and female insulation scores is also shown. The difference between insulation scores is highly significant for chrX (Wilcoxon test, p-value < 0.0001).