Fig. 1: Melanoma persister cells have altered translation activity. | Nature Communications

Fig. 1: Melanoma persister cells have altered translation activity.

From: An epitranscriptomic mechanism underlies selective mRNA translation remodelling in melanoma persister cells

Fig. 1

a Schematic of melanoma persister cell generation and subsequent analyses. Par: parental cells treated with dimethylsulfoxide (DMSO), Per: persister cells marked as red arrows. b Reversible drug tolerance of persister cells upon BRAFi/MEKi removal. Persister and parental cells were re-challenged with BRAFi/MEKi treatment on the indicated days and drug sensitivity was analysed by WST-1-based cell viability assay. c Polysome profiles of persister cells at various time points, assessed by 5–50% sucrose-gradient ultracentrifugation. Per+ represents persister cells upon continuous BRAFi/MEKi exposure. d Schematic view shows that persister cells re-acquire similar drug sensitivity as parental cells upon BRAFi/MEKi removal. Upon continuous drug exposure, persister cells can serve as a reservoir for the development of genetic acquired resistant cells. The raw data of b are available in Source Data.

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