Fig. 5: m6A methyltransferase knockdown inhibits the translation remodelling and the survival of melanoma persister cells. | Nature Communications

Fig. 5: m6A methyltransferase knockdown inhibits the translation remodelling and the survival of melanoma persister cells.

From: An epitranscriptomic mechanism underlies selective mRNA translation remodelling in melanoma persister cells

Fig. 5

a Short-term WST-1 cell viability analysis of the A375 melanoma cells expressing the indicated shRNAs upon the treatment of BRAFi/MEKi for 48 h. b, c m6A methylatransferase shRNA knockdown abrogates BRAFi/MEKi dependent persister cell-derived colony formation. An image representation (b) and the quantification on the clonogenic assay of persister cells are presented (c) (n = 3, **p-value < 0.001, unpaired t-test). d RT-qPCR quantification of mRNAs in fractions obtained by sucrose-gradient ultracentrifugation of lysates from persister cells in the presence or absence of shRNA METTL3. The raw data of a, c and d are available in Source Data.

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