Fig. 2 | Nature Communications

Fig. 2

From: Comparative analysis of squamate brains unveils multi-level variation in cerebellar architecture associated with locomotor specialization

Fig. 2

Comparative anatomy of whole-brain and cerebellar cortex. a Direct comparison of dissected (top panels) and 3D reconstructed (bottom) Pogona vitticeps brain in lateral (left panels) and dorsal (right) views. b, c Single and merged immunostainings for ZIC1/2/3 granule cell marker (green) and DAPI (blue) on sagittal sections of Anolis carolinensis (b) and Epicrates cenchria (c) cerebellar cortex. White dashed lines highlight both the cerebellum profile and part of optic tectum in contact with the cerebellum to illustrate their spatial relationships in the two species. Crossed white arrows point towards rostral (R), caudal (C), dorsal (D) and ventral (V) directions. Pial and ventricular cerebellar surfaces are indicated on their respective side. GCL, granule cell layer; ML, molecular layer; IV, fourth ventricle. Scale bars: 1 mm (a), 100 μm (b, c).

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