Fig. 1

Structure of BaPif1 complexed with a dual forked dsDNA. a DNA sequence used for crystallization: The eight out of the ten nucleotide base pairs of the central dsDNA observed in the structure are shown in violet. The brown and green blocks correspond to nucleotides bound to BaPif1 at 5′ and 3′ arms of the dual forked dsDNA. The nucleotides discussed in the text are numbered. b Structure of BaPif1 bound to dual forked dsDNA: Molecules at 5′ arms labeled as BaPif1^A and shown in two shades of brown, are related by a two-fold symmetry axis. Similarly, molecules at 3′ arms labeled as BaPif1^B and shown in two shades of green, are related by the same two-fold axis. The asymmetric unit consists of two molecules of BaPif1 (one in green and the other in brown) and one strand of DNA. ADP·AlF₄⁻ used in crystallization is shown as black sticks.