Fig. 2

Interaction of BaPif1 with forked dsDNA. Residue in brown belongs to BaPif1^A, residues in green belong to BaPif1^B and nucleotides of the forked dsDNA are in violet. Tyr91^A and Tyr412^B stack against T10. The first base pair (C11–G20) of the dsDNA is broken and C11 is stacked on one side by Ile409^B and on another side by the subsequent nucleotide G12. The other nucleotide, G20 of the broken base pair is stabilized by Val149^B and His361^B. The main chain of Lys151^B–Lys152^B anchor the phosphate backbone of the 3′ arm. A trio of residues, Phe379^B, His236^B and His377^B interact with the G12–C19 base pair of the dsDNA.