Fig. 6
From: Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases
Rapid chemical quench flow reactions to measure rate of unwinding the parental and the leading duplexes. a Single turnover unwinding assay with wtBaPif1 and E323A/K405A (b) to determine rates of unwinding. c Quantification of the unwinding reaction with wild type BaPif1. The leading duplex was unwound (circles) at 21 ± 2 s−1 and the parental duplex (squares) at 21 ± 3 s−1. d Quantification of the unwinding reaction with E323A/K405A mutant of BaPif1: The mutant unwound the leading duplex (circles) at 33 ± 1 s−1 and the parental duplex (squares) at 31 ± 3 s−1. Errors are the standard error of the fit. n = 2 independent experiments. Source gels and quantification data are provided as a Source Data file.
