Fig. 5
From: Sestrins are evolutionarily conserved mediators of exercise benefits
Sestrin regulates mitochondrial biogenesis in skeletal muscle. a Sestrin expression in different muscle tissue was analyzed by immunoblotting and band densitometry. E, extensor digitorum longus; S, soleus (SOL); P, plantaris; G, gastrocnemius (GTN); Q, quadriceps (QUAD). b Soleus muscle was analyzed by immunostaining visualizing extracellular matrix (white) and myofibers of type I (red), IIA (blue), IIB (green; SOL did not contain IIB fibers in our analysis) and IIX (black). Fiber contents of the whole cross-section were quantified (right). c–e Indicated muscle tissues were analyzed by immunoblotting and band densitometry. f, g Mitochondrial complex I (NADH-TR) and II (SDH) activities were visualized from fresh frozen sections of indicated mouse muscle. Scale bars, 100 µm. Biologically independent animals: n = 4, 2, 2 (a; Sesn1, Sesn2 and Sesn3 analyses, respectively), n = 5, 4 (b, d, e; WT and TKO, respectively), n = 4, 3 (c, PGC1α, SDHA and SDHB; WT and TKO, respectively), n = 5, 4 (c, all other proteins; WT and TKO, respectively). Error bars, s.e.m. *P < 0.05, **P < 0.01 or ***P < 0.001 from a two-tailed student’s t test. Molecular weight markers are indicated in kDa.
