Fig. 7
From: Sestrins are evolutionarily conserved mediators of exercise benefits
PGC1 is critical for endurance-extending effects of exercise and Sestrin. Flies expressing indicated transgenic components were analyzed by runspan (a, c, d, f, g) and flight performance (b, e, h) assays at indicated days. See Fig. 2 for details of how individual strains were prepared. Mef2 > dSesnWT (ON) and Con Mef2gal4 (OFF) data from the same cohort are shown as a reference. Biologically independent animal groups: n = 8, 8, 8, 8, 8, 8, 7, 7 (a, upper to lower; groups of 20 flies), n = 16 (c, f; groups of 20 flies), n = 8 (d, g; groups of 20 flies). Biologically independent animals: n = 227, 172, 164, 209, 227, 235, 138, 126 (b, left to right), n = 211, 202, 207, 196, 182, 128, 104, 133 (e, left to right), n = 211, 202, 207, 196, 138, 168, 223, 115, 146, 221, 168, 205 (h, left to right). Error bars, s.d. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 or ns, non-significant from a log-rank test (runspan) or two-tailed student’s t test (all other assays).
