Fig. 4
In situ optical characterizations of the autonomous rotation process. a Time-course CD measurement at 732 nm for clockwise autonomous rotation. For each sample, the rotor is initially fixed at position 1-1′. The times for adding removal strands r to activate the DNAzyme feet and blocking strands B1 to unlock the rotor from position 1-1′ are indicated in the plot. The arrangements of the substrates around the track are varied for different samples as illustrated in the schematic on the right (right half is shown). b Control experiment 1, in which two substrates are omitted around the ring track. Track arrangement 1-2-x–x-5 (light blue) means that there are no substrates at fh3 (fh3′) and fh4 (fh4′). c Control experiment 2, in which one substrate is omitted around the ring track. Track arrangement 1-2-3-x-5 (blue) means that there is no substrate at fh4 (fh4′). d Time-course CD measurement at 732 nm for counterclockwise autonomous rotation. The arrangements of the substrates around the track are varied for different samples as illustrated in the schematic on the right (left half is shown). The experiments were carried out on 14 samples, source data are provided with the paper.
