Fig. 4
From: A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice
UVRAGFS exacerbates intestinal colitis in vivo. a Kaplan–Meier curve showing survival of control and iUVRAGFS mice (n = 10 per group) fed with 5% DSS in drinking water for 6 days. **P < 0.01 (Log-rank test). b Disease activity index (DAI) of colitis in control and iUVRAGFS mice in a. The DAI value was calculated as described in Methods. c Colon lengths of DSS-treated mice with indicated genotypes were assessed at the time of necropsy (days 6 and 10) with representative macroscopic images (left). d H&E-stained sections of colon from DSS-treated mice with indicated genotypes on day 10 after DSS. Inset magnification highlights inflammation and crypt damage. Arrows indicate neutrophil infiltration. Scale bars, 300 μm. e Histopathology scores as indicated in the colon of mice with indicated genotypes after DSS-induced colitis. n = 5–6 per genotype. f Co-IP of inflammasome complex assembly as indicated in colon tissues of mice with indicated genotypes before/after DSS. WB of indicated protein expression in colon homogenates are shown (lower panel). g, h Densitometric quantification of the ratios of LC3-II/LC3-I and p62/actin (g) and the relative production of cleaved caspase-1, IL-1β, and IL-18 to their precursors (h) in the colons in f. n = 3 (g) and 4 (h) independent experiments. i ELISA of IL-1β, IL-6, and TNF-α (left panel) and IL-18 (right panel) in colons of mice with indicated genotypes after DSS treatment. n = 3–5 per genotype. j, k Histopathology scores (j) and H&E-stained sections (k) of colons from mice with indicated genotypes treated with MCC950 during DSS-induced colitis. n = 3–6 per genotype. Scale bars, 100 μm. l ELISA of cytokines in colons of mice with indicated genotypes in j. n = 3–5 per genotype. Data in f are from one experiment that is representative of three independent experiments. For all quantifications, data (mean ± SD) were analyzed with Student’s t-test or two-way ANOVA. n.s., not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ***P < 0.0001. Source data are provided as a Source Data file. See Supplementary Fig. 9 for uncropped data of f.
