Fig. 2: Sensory innervation in endplates correlates with increase of osteoclasts in LSI model. | Nature Communications

Fig. 2: Sensory innervation in endplates correlates with increase of osteoclasts in LSI model.

From: Sensory innervation in porous endplates by Netrin-1 from osteoclasts mediates PGE2-induced spinal hypersensitivity in mice

Fig. 2

a Representative images of coronal mouse caudal endplate sections of L4/5 stained for TRAP (magenta) at 2, 4, and 8 weeks after LSI or sham surgery. Scale bars, 50 μm. b Quantitative analysis of the number of TRAP+ cells in endplates. c Representative immunofluorescent images of CGRP+ sensory nerve fibers (red) and DAPI (blue) staining of nuclei in mouse caudal endplates of L4/5 at 2, 4, and 8 weeks after LSI or sham surgery. Scale bars, 50 μm. d Percentage of CGRP+ area in endplates. e Representative images of immunofluorescent analysis of CGRP+ (red), PGP9.5+ (green) nerve fibers, and DAPI (blue) staining of nuclei in mouse caudal endplates of L4/5 at 8 weeks after LSI or sham surgery. Scale bars, 50 μm. f Representative images of immunofluorescent analysis of IB4+ (green) sensory nerve fibers and DAPI (blue) staining of nuclei in mouse caudal endplates of L4/5 at 2, 4, and 8 weeks after LSI or sham surgery. Scale bars, 100 μm. **p < 0.01 compared with the sham surgery mice at the corresponding time points. n = 6 per group (b, d). Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file.

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