Fig. 3
Identification of lilli as a strong genetic suppressor of poly(GR) toxicity. a The design of a genetic deficiency screen in Drosophila. Def, deficiency lines. b Deficiencies Df(2L)C144, Df(2)ED4651, and Df(2)BSC180 in trans significantly extended the lifespan of (GR)80 flies. The n number for each genotype is in the figure. p < 0.0001 (log-rank test). c Survival analysis of (GR)80 flies that are heterozygous for different lilli mutant alleles. Partial loss of Lilli activity by lilliA17-2, lilli4U5, and lillik05431 heterozygosity extended median survival by 14, 8, and 7 days, respectively. p < 0.0001 (log-rank test). Lilli overexpression decreased the median survival by 3 days. p < 0.0001 (log-rank test). The n number for each genotype is in the figure. d Effect of partial loss of Lilli activity on lifespan of control flies. The n number for each genotype is in the figure. e Reducing Lilli function rescues the climbing defect of (GR)80 flies in the negative geotaxis assay. One dot = 20 flies, n = 22, 5, 4, and 5 assays for genotypes from the left to the right, respectively. ****p < 0.0001 (one-way ANOVA, Dunnett’s test). f Overexpression of Lilli enhanced the climbing defect of (GR)80 flies. One dot = 20 flies, n = 5, 4, 5, 4 assays for genotypes from the left to the right, respectively. *p < 0.05; **p < 0.01 (Welch’s test). Source data are provided as a Source Data file.
