Fig. 1: Study design and general overview of detected metabolites. | Nature Communications

Fig. 1: Study design and general overview of detected metabolites.

From: Metabolomics analysis of human acute graft-versus-host disease reveals changes in host and microbiota-derived metabolites

Fig. 1

a Metabolomic profile was studied in two cohorts of patients, a single center cohort from Saint Louis hospital, Paris (cohort 1), and a multicentric French cohort (cohort 2). Plasma samples were collected in sibling donors before stem cell collection and in recipients after transplantation, at day 90 for those who did not developed GvHD or before corticosteroid treatment at disease onset for those who developed GvHD. Samples were frozen and then analyzed by ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for circulating metabolites identification and quantification. *One donor did not give his consent for further analysis. b In all, 801 and 923 metabolites were identified in cohorts 1 and 2, respectively, with 653 metabolites shared by both cohorts. c Treemap showing the distribution of metabolites among super pathway (in color) and their related sub-pathways (square) for the 653 metabolites used for statistical analysis. Each number corresponds to one of the sub-pathway listed in Source Data file.

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