Fig. 1: Activation of TLR5 by cagPAI-positive, but not cagPAI-negative H. pylori strains.
From: T4SS-dependent TLR5 activation by Helicobacter pylori infection
a H. pylori type-I, but not type-II strains, express a functional T4SS allowing for CagA phosphorylation in infected AGS cells as control. b TLR5 activation as quantified by NF-κB luciferase reporter gene assay is restricted to type-I isolates in HEK reporter cells. c CagA delivery and its phosphorylation in AGS cells require functional cagL and cagA, but not vacA, ureB and flaA genes. d TLR5 activation in HEK293 cells requires functional cagL. Recombinant flagellin (rFliC) of Salmonella was used as positive control. e, CagL and rFliC interact with TLR5 on TLR5+ cells as determined by immunoprecipitation with α-TLR5 antibodies. Quantitative data are shown as means ± SD. ****p < 0.0001 (one-way ANOVA). Each red dot represents a single data point. Source data are provided as a Source Data file.
