Fig. 2: The T4SS-pilus protein CagL of H. pylori interacts directly with TLR5.
From: T4SS-dependent TLR5 activation by Helicobacter pylori infection
a Binding of FITC-labeled H. pylori G27 WT or ΔcagL mutant to parental and TLR5+ cells, as monitored over time using the LigandTracer system under live cell conditions. b Peptide arrays of overlapping CagL 15-mer peptides were incubated with recombinant His-tagged TLR5. Probing with α-His antibodies revealed binding of TLR5 to peptide-20, but not the partially overlapping peptides-19 or −21. c The peptide-20 sequence of CagL shows homology to the flagellin αD1a domains of TLR5-activating, but not TLR5-non-activating flagellins. Quantitative data are shown as means ± SD. **p < 0.01; ***p < 0.001 (two-way ANOVA). Source data are provided as a Source Data file.
